Cell Biology, Protein Biology & Immunology
Cell Biology
In vitro Drug Screening Assays using Mammalian Cells
- Cancer (Cytotoxicity, Proliferation, Apoptosis)
- Diabetes (Glucose uptake, enzyme assays, insulin signaling pathway)
- Inflammatory Disorders (Pro & anti-inflammatory cytokines)
Development of Cell Lines
- Stable Transfected Cell Lines
- Transient Transfected Cell Lines
Cell Sorting & Analysis (FACSCalibur, FACSAria, autoMACS)
- Cell Sorting
- Stem Cell / Immune Cell Characterization
- Cell Cycle Analysis
- Cell Migration & Proliferation assays
- Mitochondrial Membrane Potential
- Detection of micronuclei
- Apoptotic analysis
Protein Biology
Characterization of Proteins
- Extraction and Quantification of Total Proteins from Cells & Tissues
- Molecular Weight Determination by SDS-PAGE Analysis
Assessment of Protein Expression
- SDS – PAGE & Immunoblot Analysis
Cloning & Expression of Proteins
- Scanning of Gels (Agarose, SDS & 2D-Gels) & Membranes using Typhoon FLA 9500 Biomolecular Imager
Documentation of Gels & Blots
- Bacterial Cloning and Transformation
- Transfection into Mammalian Cells
Immunoassays
- Single-plex ELISA using Multi-mode Microplate Reader
- Multiplex ELISA using CiraScan Imager
- Multiple Bioanalyte Quantification using LUMINEX 200 Microbead-based Analysis
Biochemical Assays
- Membrane Stabilization Assay
- Inhibition of Albumin Denaturation Assay
- Protease Inhibition Assay
Anti-oxidant Screening
- Oxygen Radical Absorbing Capacity (ORAC) Determination Assay
- Thiobarbituricacid Reducing Substances (TBARS) Assay
- DPPH Assay
- ABTS Assay